Spectroscopy courses

spectrioscopy

Ultraviolet Spectroscopy

A one-day course on the fundamentals of UV spectroscopy including:

  • The electromagnetic spectrum
  • Bonding orbital energy levels, pi bonds and conjugation
  • Electronic transitions and absorbance wavelengths
  • The Beer-Lambert Law
  • Spectrometer components and optical path designs
  • Sample cuvettes – selection, use and cleaning
  • Qualitative and quantitative analysis
  • Derivative spectroscopy
  • Typical applications
  • Practical exercise – instrument calibration and assay of an unknown sample

Fourier Transform Infrared Spectroscopy

A one-day course on the fundamentals of IR spectroscopy including:

  • The electromagnetic spectrum
  • Bond stretching, bending and rotation
  • Frequency, wavelength, wavenumber and band intensity
  • Absorbance regions associated with different functional groups
  • Spectrometer components and optical path designs
  • The interferogram
  • Sample preparation – thin film, KBr pellet and diamond ATR
  • Artefacts caused by carbon dioxide and water
  • Qualitative and quantitative analysis
  • Typical applications
  • Practical exercise – sample preparation and data acquisition

Interpretation of Infrared Spectra

A one-day course on the interpretation of IR spectra including:

  • Revision of modes of absorbance in the IR region
  • Appearance of typical functional group absorbance bands – spectral region and intensity
  • Positional isomers, including aromatic substitution patterns
  • Practical exercise – interpretation of unknown spectra

Mass Spectrometry

A one-day course on the fundamentals of mass spectrometry including:

  • The covalent bond
  • Bond strength and fragmentation
  • Deflection of ions in a magnetic field and mass:charge ratio
  • Components of a mass spectrometer – overview
  • Types of ion source, mass analyser and detector
  • Stable isotopes and monoisotopic masses of common elements
  • Mass resolution
  • Molecular formulae from accurate mass data
  • Large molecules and multiple charging
  • Interfaces for GC-MS and LC-MS
  • Practical exercise – sample preparation and data acquisition

Interpretation of Electron Ionisation Mass Spectra

A one-day course on the interpretation of mass spectra including:

  • Revision of basic theory and instrument design
  • Data produced by different ionisation methods and mass analysers
  • Typical EI mass spectra and common neutral losses
  • Identifying possible molecular ions
  • The odd nitrogen rule
  • Rings plus double bonds
  • Stable isotope patterns and molecular composition
  • Data from accurate mass determinations
  • Fragmentation and rearrangement mechanisms
  • Worked examples
  • Practical exercise – interpretation of unknown spectra